fibroblast hfl 1 Search Results


human fetal lung fibroblast hfl 1 cells  (ATCC)
94
ATCC human fetal lung fibroblast hfl 1 cells
Human Fetal Lung Fibroblast Hfl 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human fetal lung fibroblast hfl 1 cells/product/ATCC
Average 94 stars, based on 1 article reviews
human fetal lung fibroblast hfl 1 cells - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
human lung fibroblast hfl1 cells  (JCRB Cell Bank)
90
JCRB Cell Bank human lung fibroblast hfl1 cells
Osa-miR172d-5p identified as a plant miR candidate with an anti-fibrotic effect. ( A ) Scheme of plant miR selection. ( B ) In silico analysis of the interaction between osa-miR172d-5p and TAB1. ( C ) Human lung <t>fibroblast</t> <t>HFL1</t> cells were transfected with the osa-miR172d-5p for 48 h, and TAB1 expression was evaluated via western blot analysis ( n = 4). ( D ) HFL1 cells were transfected with the indicated concentration of osa-miR172d-5p for 48 h, and cDNA was evaluated via qRT-PCR ( n = 4). Data are shown as mean ± SEM. * P < 0.05. *** P < 0.001 versus control group.
Human Lung Fibroblast Hfl1 Cells, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human lung fibroblast hfl1 cells/product/JCRB Cell Bank
Average 90 stars, based on 1 article reviews
human lung fibroblast hfl1 cells - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
human foetal lung fibroblast 1 (hfl1) cell line  (BioVector NTCC)
90
BioVector NTCC human foetal lung fibroblast 1 (hfl1) cell line
Osa-miR172d-5p identified as a plant miR candidate with an anti-fibrotic effect. ( A ) Scheme of plant miR selection. ( B ) In silico analysis of the interaction between osa-miR172d-5p and TAB1. ( C ) Human lung <t>fibroblast</t> <t>HFL1</t> cells were transfected with the osa-miR172d-5p for 48 h, and TAB1 expression was evaluated via western blot analysis ( n = 4). ( D ) HFL1 cells were transfected with the indicated concentration of osa-miR172d-5p for 48 h, and cDNA was evaluated via qRT-PCR ( n = 4). Data are shown as mean ± SEM. * P < 0.05. *** P < 0.001 versus control group.
Human Foetal Lung Fibroblast 1 (Hfl1) Cell Line, supplied by BioVector NTCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human foetal lung fibroblast 1 (hfl1) cell line/product/BioVector NTCC
Average 90 stars, based on 1 article reviews
human foetal lung fibroblast 1 (hfl1) cell line - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
human embryonic fibroblast cell line hfl1  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection human embryonic fibroblast cell line hfl1
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Embryonic Fibroblast Cell Line Hfl1, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human embryonic fibroblast cell line hfl1/product/China Center for Type Culture Collection
Average 90 stars, based on 1 article reviews
human embryonic fibroblast cell line hfl1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
human embryonic lung fibroblasts (hfl1) cell  (Galectin Therapeutics)
90
Galectin Therapeutics human embryonic lung fibroblasts (hfl1) cell
Galectin-1 overexpression in <t>HFL1</t> cells. (a) Fluorescence images presented that galectin-1 was successfully overexpression in HFL1 cells (magnification: ×100). (b) qRT-PCR was conducted to measure galectin-1 mRnas expression. (c) Western blotting was conducted to measure galectin-1, TGF-β1 proteins. (d) The qualification of galectin-1, TGF-β1 proteins expression in ctrl and galectin-1-OE groups at different time. (e) Western blotting was conducted to measure α-SMA proteins expression in ctrl and galectin-1-OE groups at different time. The quantitative analysis of galectin-1 (f), TGF-β1 (g), and α-SMA (h) proteins. * p < .05, ** p < .01, *** p < .001.
Human Embryonic Lung Fibroblasts (Hfl1) Cell, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human embryonic lung fibroblasts (hfl1) cell/product/Galectin Therapeutics
Average 90 stars, based on 1 article reviews
human embryonic lung fibroblasts (hfl1) cell - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
lung (hfl1) fibroblasts  (Macquarie Bank)
90
Macquarie Bank lung (hfl1) fibroblasts
Galectin-1 overexpression in <t>HFL1</t> cells. (a) Fluorescence images presented that galectin-1 was successfully overexpression in HFL1 cells (magnification: ×100). (b) qRT-PCR was conducted to measure galectin-1 mRnas expression. (c) Western blotting was conducted to measure galectin-1, TGF-β1 proteins. (d) The qualification of galectin-1, TGF-β1 proteins expression in ctrl and galectin-1-OE groups at different time. (e) Western blotting was conducted to measure α-SMA proteins expression in ctrl and galectin-1-OE groups at different time. The quantitative analysis of galectin-1 (f), TGF-β1 (g), and α-SMA (h) proteins. * p < .05, ** p < .01, *** p < .001.
Lung (Hfl1) Fibroblasts, supplied by Macquarie Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lung (hfl1) fibroblasts/product/Macquarie Bank
Average 90 stars, based on 1 article reviews
lung (hfl1) fibroblasts - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Osa-miR172d-5p identified as a plant miR candidate with an anti-fibrotic effect. ( A ) Scheme of plant miR selection. ( B ) In silico analysis of the interaction between osa-miR172d-5p and TAB1. ( C ) Human lung fibroblast HFL1 cells were transfected with the osa-miR172d-5p for 48 h, and TAB1 expression was evaluated via western blot analysis ( n = 4). ( D ) HFL1 cells were transfected with the indicated concentration of osa-miR172d-5p for 48 h, and cDNA was evaluated via qRT-PCR ( n = 4). Data are shown as mean ± SEM. * P < 0.05. *** P < 0.001 versus control group.

Journal: Scientific Reports

Article Title: Plant miRNA osa-miR172d-5p suppressed lung fibrosis by targeting Tab1

doi: 10.1038/s41598-023-29188-6

Figure Lengend Snippet: Osa-miR172d-5p identified as a plant miR candidate with an anti-fibrotic effect. ( A ) Scheme of plant miR selection. ( B ) In silico analysis of the interaction between osa-miR172d-5p and TAB1. ( C ) Human lung fibroblast HFL1 cells were transfected with the osa-miR172d-5p for 48 h, and TAB1 expression was evaluated via western blot analysis ( n = 4). ( D ) HFL1 cells were transfected with the indicated concentration of osa-miR172d-5p for 48 h, and cDNA was evaluated via qRT-PCR ( n = 4). Data are shown as mean ± SEM. * P < 0.05. *** P < 0.001 versus control group.

Article Snippet: Human lung fibroblast HFL1 cells (JCRB, Osaka, Japan) were cultured in 10% fetal bovine serum (FBS) (Sigma-Aldrich), Dulbecco's Modified Eagle Medium (DMEM) (044-29765; Fujifilm Tokyo, Japan) supplemented with penicillin G (876111)–streptomycin (876161; Meiji Pharmaceutical Co., Tokyo, Japan) in 5% CO 2 and 100% humidity at 37 °C.

Techniques: Selection, In Silico, Transfection, Expressing, Western Blot, Concentration Assay, Quantitative RT-PCR, Control

TAB1 knockdown suppressed TGFβ-induced fibrotic gene expression. ( A ) Human lung fibroblast HFL1 cells were transfected with TAB1-siRNA (10 nM, 48 h) and TAB1 expression levels were determined by western blot analysis. ( B – D ) Human lung fibroblast HFL1 cells were transfected with TAB1-siRNA (10 nM, 48 h) and treated with TGFβ (5 ng/mL for B , D 48 h; C 24 h). mRNA expression levels were assessed via RT-qPCR. ( B ) ASMA (αSMA) (n = 4), ( C ) COL1A1 (n = 4), and ( D ) FN (fibronectin; n = 4). Data are shown as mean ± SEM. * P < 0.05. ** P < 0.01. *** P < 0.001 versus control group.

Journal: Scientific Reports

Article Title: Plant miRNA osa-miR172d-5p suppressed lung fibrosis by targeting Tab1

doi: 10.1038/s41598-023-29188-6

Figure Lengend Snippet: TAB1 knockdown suppressed TGFβ-induced fibrotic gene expression. ( A ) Human lung fibroblast HFL1 cells were transfected with TAB1-siRNA (10 nM, 48 h) and TAB1 expression levels were determined by western blot analysis. ( B – D ) Human lung fibroblast HFL1 cells were transfected with TAB1-siRNA (10 nM, 48 h) and treated with TGFβ (5 ng/mL for B , D 48 h; C 24 h). mRNA expression levels were assessed via RT-qPCR. ( B ) ASMA (αSMA) (n = 4), ( C ) COL1A1 (n = 4), and ( D ) FN (fibronectin; n = 4). Data are shown as mean ± SEM. * P < 0.05. ** P < 0.01. *** P < 0.001 versus control group.

Article Snippet: Human lung fibroblast HFL1 cells (JCRB, Osaka, Japan) were cultured in 10% fetal bovine serum (FBS) (Sigma-Aldrich), Dulbecco's Modified Eagle Medium (DMEM) (044-29765; Fujifilm Tokyo, Japan) supplemented with penicillin G (876111)–streptomycin (876161; Meiji Pharmaceutical Co., Tokyo, Japan) in 5% CO 2 and 100% humidity at 37 °C.

Techniques: Knockdown, Gene Expression, Transfection, Expressing, Western Blot, Quantitative RT-PCR, Control

Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with HFL1 cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01

Journal: Journal of Cellular and Molecular Medicine

Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner

doi: 10.1111/jcmm.16524

Figure Lengend Snippet: Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with HFL1 cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01

Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and human embryonic fibroblast cell line HFL1 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Derivative Assay, In Vitro, Incubation, Western Blot, Quantitative RT-PCR

SiO 2 ‐Exos induce lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) treated with various exosomes (SiO 2 ‐Exos or SiO 2 + GW4869‐Exos). Two‐way ANOVA; ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant

Journal: Journal of Cellular and Molecular Medicine

Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner

doi: 10.1111/jcmm.16524

Figure Lengend Snippet: SiO 2 ‐Exos induce lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) treated with various exosomes (SiO 2 ‐Exos or SiO 2 + GW4869‐Exos). Two‐way ANOVA; ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant

Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and human embryonic fibroblast cell line HFL1 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Migration, CCK-8 Assay, Wound Closure Assay

SiO 2 ‐Exo‐induced myofibroblast differentiation is ER stress dependent (A‐C, E) Western blot analysis of BIP, XBP1s, P ‐eIF2α, α‐SMA, Collagen I, and GAPDH in HFL1 cells (A, C) and NIH‐3T3 cells (B, E) incubated with SiO 2 ‐Exos (± 4‐PBA) at different points in time (0, 8, 16 or 24 h). (D, F) Analysis of collagen I, α‐SMA, and GAPDH in HFL1 cells (D) and NIH‐3T3 cells (F) incubated with SiO 2 ‐Exos or SiO 2 ‐Exos +4‐PBA for 24 h

Journal: Journal of Cellular and Molecular Medicine

Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner

doi: 10.1111/jcmm.16524

Figure Lengend Snippet: SiO 2 ‐Exo‐induced myofibroblast differentiation is ER stress dependent (A‐C, E) Western blot analysis of BIP, XBP1s, P ‐eIF2α, α‐SMA, Collagen I, and GAPDH in HFL1 cells (A, C) and NIH‐3T3 cells (B, E) incubated with SiO 2 ‐Exos (± 4‐PBA) at different points in time (0, 8, 16 or 24 h). (D, F) Analysis of collagen I, α‐SMA, and GAPDH in HFL1 cells (D) and NIH‐3T3 cells (F) incubated with SiO 2 ‐Exos or SiO 2 ‐Exos +4‐PBA for 24 h

Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and human embryonic fibroblast cell line HFL1 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Western Blot, Incubation

Inhibition of ER stress attenuates SiO 2 ‐Exo‐induced lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) incubated with SiO 2 ‐Exos ± 4‐PBA. Two‐way ANOVA; * P < .05, ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant

Journal: Journal of Cellular and Molecular Medicine

Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner

doi: 10.1111/jcmm.16524

Figure Lengend Snippet: Inhibition of ER stress attenuates SiO 2 ‐Exo‐induced lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) incubated with SiO 2 ‐Exos ± 4‐PBA. Two‐way ANOVA; * P < .05, ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant

Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and human embryonic fibroblast cell line HFL1 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Inhibition, Migration, CCK-8 Assay, Incubation, Wound Closure Assay

Galectin-1 overexpression in HFL1 cells. (a) Fluorescence images presented that galectin-1 was successfully overexpression in HFL1 cells (magnification: ×100). (b) qRT-PCR was conducted to measure galectin-1 mRnas expression. (c) Western blotting was conducted to measure galectin-1, TGF-β1 proteins. (d) The qualification of galectin-1, TGF-β1 proteins expression in ctrl and galectin-1-OE groups at different time. (e) Western blotting was conducted to measure α-SMA proteins expression in ctrl and galectin-1-OE groups at different time. The quantitative analysis of galectin-1 (f), TGF-β1 (g), and α-SMA (h) proteins. * p < .05, ** p < .01, *** p < .001.

Journal: Cell Adhesion & Migration

Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer

doi: 10.1080/19336918.2024.2335881

Figure Lengend Snippet: Galectin-1 overexpression in HFL1 cells. (a) Fluorescence images presented that galectin-1 was successfully overexpression in HFL1 cells (magnification: ×100). (b) qRT-PCR was conducted to measure galectin-1 mRnas expression. (c) Western blotting was conducted to measure galectin-1, TGF-β1 proteins. (d) The qualification of galectin-1, TGF-β1 proteins expression in ctrl and galectin-1-OE groups at different time. (e) Western blotting was conducted to measure α-SMA proteins expression in ctrl and galectin-1-OE groups at different time. The quantitative analysis of galectin-1 (f), TGF-β1 (g), and α-SMA (h) proteins. * p < .05, ** p < .01, *** p < .001.

Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

Techniques: Over Expression, Fluorescence, Quantitative RT-PCR, Expressing, Western Blot

Galectin-1 overexpression promotes tumor growth in vivo . (a) Imaging technology to monitor the growth of A549 ×enograft tumors. (b)The growth of xenograft tumors was determined by volume. (c) The growth of xenograft tumors was determined by tumor weight. vs A549 group, ** p < .01; vs A549+Anlotinib group, ## p < .01; vs gal-1 OE@HFL1:A549 group, && p < .01.

Journal: Cell Adhesion & Migration

Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer

doi: 10.1080/19336918.2024.2335881

Figure Lengend Snippet: Galectin-1 overexpression promotes tumor growth in vivo . (a) Imaging technology to monitor the growth of A549 ×enograft tumors. (b)The growth of xenograft tumors was determined by volume. (c) The growth of xenograft tumors was determined by tumor weight. vs A549 group, ** p < .01; vs A549+Anlotinib group, ## p < .01; vs gal-1 OE@HFL1:A549 group, && p < .01.

Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

Techniques: Over Expression, In Vivo, Imaging

IHC detected α-SMA, and Ki67 protein expression in vivo . (a) IHC staining of α-SMA protein in xenograft tumors. (b) The staining index of α-SMA. (c) IHC staining of Ki67 protein in xenograft tumors. (d) Positive cell percent of Ki67. Scale bar: 50 μm. (e) Western blotting was conducted to measure MMP2, MMP9, TGF-β1, VEGFA, PDGFA. (f) The qualification of proteins expression in different groups. vs A549 group, * p < .05, ** p < .01; vs A549+Anlotinib group, # p < .05, ## p < .01; vs gal-1 OE@HFL1:A549 group, & p < .05, && p < .01.

Journal: Cell Adhesion & Migration

Article Title: Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer

doi: 10.1080/19336918.2024.2335881

Figure Lengend Snippet: IHC detected α-SMA, and Ki67 protein expression in vivo . (a) IHC staining of α-SMA protein in xenograft tumors. (b) The staining index of α-SMA. (c) IHC staining of Ki67 protein in xenograft tumors. (d) Positive cell percent of Ki67. Scale bar: 50 μm. (e) Western blotting was conducted to measure MMP2, MMP9, TGF-β1, VEGFA, PDGFA. (f) The qualification of proteins expression in different groups. vs A549 group, * p < .05, ** p < .01; vs A549+Anlotinib group, # p < .05, ## p < .01; vs gal-1 OE@HFL1:A549 group, & p < .05, && p < .01.

Article Snippet: Galectin-1 overexpression was conducted in Human embryonic lung fibroblasts (HFL1) cell.

Techniques: Expressing, In Vivo, Immunohistochemistry, Staining, Western Blot